![]() ![]() |
![]() |
|||||||||||||||||||||||
|
Aquatic disposal of slurried wastes from coal combustion has recently been implicated by our research group as a potential threat to the health of amphibian populations. Approximately 57 million tons of coal combustion wastes (CCW), which contain high concentrations of trace elements including As, Cd, and Se, are discharged into aquatic settling basins in the United States each year. Field surveys in one such disposal system located adjacent to the Savannah River in SC, USA revealed that at least 14 species of anurans utilize the 86 ha site during their respective breeding seasons. Published field surveys conducted by our research group and colleagues (C. Rowe) revealed that bullfrog (Rana catesbeiana) larvae sampled from the system accumulate high concentrations of trace elements and exhibit developmental abnormalities, increased energy expenditures, and altered swimming performance (see publication list). Moreover, transplantations of eggs of the southern toad (Bufo terrestris) to the contaminated system suggest that reduced reproductive success or complete reproductive failure may result from amphibians being attracted to CCW-contaminated breeding sites (Rowe et al., 2001; SREL reprint 2517).
|
|||||||||||||||||||||||
![]() |
|||||||||||||||||||||||
| Discharge of effluent contaminated with coal combustion wastes. | |||||||||||||||||||||||
![]() |
|||||||||||||||||||||||
![]() |
|||||||||||||||||||||||
| Working with Joel Snodgrass at Towson University, we have taken the necessary next step of establishing a definitive link between CCW-exposure and developmental abnormalities by conducting controlled laboratory studies. We exposed green frog (Rana clamitans) and wood frog (R. sylvatica) larvae to either clean sand or CCW-contaminated sediment to investigate the direct effects of CCW exposure on trace element accumulation, growth, developmental rate, malformations, survival, and metamorphic success. While both species accumulated significant concentrations of at least six trace elements (As, Cd, Fe, Se, Sr, and V), effects of exposure to CCW varied between species, with R. clamitans larvae experiencing more severe effects including a 26% reduction in survival and a 45 % reduction in metamorphic success. Furthermore, exposure to CCW decreased growth and developmental rates among larvae of both species that successfully completed metamorphosis. Larval period duration was increased by 10% and 11%, and size at metamorphosis was decreased by10% and 39% in R. clamitans and R. sylvatica exposed to CCW, respectively. Rates of malformations were = 4%, and were not dependent on species or sediment treatment. Our results confirm the direct effects of CCW on aquatic amphibian larvae suggested by our previous field studies, and indicate that considerable variation may exist in sensitivity among species exposed to CCW. It is possible that more pronounced adverse effects occurred in Rana clamitans as a result of its longer and more variable larval period compared to R sylvatica. However, variation in species sensitivity could also relate to inherent phylogenetic differences in physiology similar to inter-species (and even inter-population) variation described by our colleague Tina Bridges. | |||||||||||||||||||||||
| Bullfrog tadpoles raised in a control habitat (A) and in an area contaminated with coal combustion wastes (B & C). | |||||||||||||||||||||||
![]() |
|||||||||||||||||||||||
| Green frog (Rana clamitans). | |||||||||||||||||||||||
![]() |
|||||||||||||||||||||||
![]() |
![]() |
||||||||||||||||||||||
| Percent of Rana clamitans and R. sylvatica larvae exposed to clean sand (control) or CCW-contaminated sediment (CCW) that completed metamorphosis before the end of a 195 or 39-day exposure period, respectively. Error bars are +/- 1 SE. | |||||||||||||||||||||||
| Mean mass at stage (A) and days to stage (B) of Rana clamitans and R. sylvatica larvae exposed to either clean sand (control) or CCW-contaminated sediment (CCW). Error bars are +/- 1 SE. | |||||||||||||||||||||||
| back to amphibian research page | |||||||||||||||||||||||
|
|
|||||||||||||||||||||||
| program home | people | facilities | research | publications | collaborators | student information |FiW Home | |||||||||||||||||||||||